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Mapping and characterization of the new adult plant leaf rust resistance gene Lr77 derived from Santa Fe winter wheat

Kolmer, JamesA., Su, Zhenqi, Bernardo, Amy, Bai, Guihua, Chao, Shiaoman
Theoretical and applied genetics 2018 v.131 no.7 pp. 1553-1560
Puccinia recondita, Triticum aestivum, alleles, backcrossing, chromosome mapping, chromosomes, cultivars, disease resistance, germplasm, inbred lines, iron, leaf rust, mature plants, plant pathogenic fungi, polymerase chain reaction, quantitative trait loci, resistance genes, single nucleotide polymorphism, winter, winter wheat
KEY MESSAGE: A new gene for adult plant leaf rust resistance in wheat was mapped to chromosome 3BL. This gene was designated as Lr77. ‘Santa Fe’ is a hard red winter cultivar that has had long-lasting resistance to the leaf rust fungus, Puccinia triticina. The objective of this study was to determine the chromosome location of the adult plant leaf rust resistance in Santa Fe wheat. A partial backcross line of ‘Thatcher’ (Tc) wheat with adult plant leaf rust resistance derived from Santa Fe was crossed with Thatcher to develop a Thatcher//Tc*2/Santa Fe F₆ recombinant inbred line (RIL) population. The RIL population and parental lines were evaluated for segregation of leaf rust resistance in three field plot tests and in an adult plant greenhouse test. A genetic map of the RIL population was constructed using 90,000 single-nucleotide polymorphism (SNP) markers with the Illumina Infinium iSelect 90K wheat bead array. A significant quantitative trait locus for reduction of leaf rust severity in all four tests was found on chromosome 3BL that segregated as a single adult plant resistance gene. The RILs with the allele from the resistant parent for SNP marker IWB10344 had lower leaf rust severity and a moderately resistant to moderately susceptible response compared to the susceptible RILs and Thatcher. The gene derived from Santa Fe on chromosome 3BL was designated as Lr77. Kompetitive allele-specific polymerase chain reaction assay markers linked to Lr77 on 3BL should be useful for selection of wheat germplasm with this gene.