Main content area

A novel approach for toluene gas treatment using a downflow hanging sponge reactor

Yamaguchi, Tsuyoshi, Nakamura, Syoichiro, Hatamoto, Masashi, Tamura, Eisuke, Tanikawa, Daisuke, Kawakami, Shuji, Nakamura, Akinobu, Kato, Kaoru, Nagano, Akihiro, Yamaguchi, Takashi
Applied microbiology and biotechnology 2018 v.102 no.13 pp. 5625-5634
Pseudomonas putida, Pseudoxanthomonas spadix, bacteria, bioreactors, clones, genes, industrial applications, liquids, models, ribosomal RNA, toluene, total suspended solids, volatile organic compounds
A novel gas-scrubbing bioreactor based on a downflow hanging sponge (DHS) reactor was developed as a new volatile organic compound (VOC) treatment system. In this study, the effects of varying the space velocity and gas/liquid ratio were investigated to assess the effectiveness of using toluene gas as a model VOC. Under optimal conditions, the toluene removal rate was greater than 80%, and the maximum elimination capacity was observed at approximately 13 g-C m⁻³ h⁻¹. The DHS reactor demonstrated slight pressure loss (20 Pa) and a high concentration of suspended solids (up to 30,000 mg/L-sponge). Cloning analysis of the 16S rRNA and functional genes of toluene degradation pathways (tmoA, todC, tbmD, xylA, and bssA) revealed that the clones belonging to the toluene-degrading bacterium Pseudomonas putida constituted the predominant species detected at the bottom of the DHS reactor. The toluene-degrading bacteria Pseudoxanthomonas spadix and Pseudomonas sp. were also detected by tmoA- and todC-targeted cloning analyses, respectively. These results demonstrate the potential for the industrial application of this novel DHS reactor for toluene gas treatment.