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A novel approach for toluene gas treatment using a downflow hanging sponge reactor

Author:
Yamaguchi, Tsuyoshi, Nakamura, Syoichiro, Hatamoto, Masashi, Tamura, Eisuke, Tanikawa, Daisuke, Kawakami, Shuji, Nakamura, Akinobu, Kato, Kaoru, Nagano, Akihiro, Yamaguchi, Takashi
Source:
Applied microbiology and biotechnology 2018 v.102 no.13 pp. 5625-5634
ISSN:
0175-7598
Subject:
Pseudomonas putida, Pseudoxanthomonas spadix, bacteria, bioreactors, clones, genes, industrial applications, liquids, models, ribosomal RNA, toluene, total suspended solids, volatile organic compounds
Abstract:
A novel gas-scrubbing bioreactor based on a downflow hanging sponge (DHS) reactor was developed as a new volatile organic compound (VOC) treatment system. In this study, the effects of varying the space velocity and gas/liquid ratio were investigated to assess the effectiveness of using toluene gas as a model VOC. Under optimal conditions, the toluene removal rate was greater than 80%, and the maximum elimination capacity was observed at approximately 13 g-C m⁻³ h⁻¹. The DHS reactor demonstrated slight pressure loss (20 Pa) and a high concentration of suspended solids (up to 30,000 mg/L-sponge). Cloning analysis of the 16S rRNA and functional genes of toluene degradation pathways (tmoA, todC, tbmD, xylA, and bssA) revealed that the clones belonging to the toluene-degrading bacterium Pseudomonas putida constituted the predominant species detected at the bottom of the DHS reactor. The toluene-degrading bacteria Pseudoxanthomonas spadix and Pseudomonas sp. were also detected by tmoA- and todC-targeted cloning analyses, respectively. These results demonstrate the potential for the industrial application of this novel DHS reactor for toluene gas treatment.
Agid:
5964381