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UV-B susceptibility and photoreactivation in embryonic development of the two-spotted spider mite, Tetranychus urticae

Author:
Yoshioka, Yoshio, Gotoh, Tetsuo, Suzuki, Takeshi
Source:
Experimental & applied acarology 2018 v.75 no.2 pp. 155-166
ISSN:
0168-8162
Subject:
DNA damage, Tetranychus urticae, acarology, eggs, embryogenesis, irradiation, morphogenesis, oviposition, pests, physical control, ultraviolet radiation
Abstract:
Developmental errors are often induced in the embryos of many organisms by environmental stress. Ultraviolet-B radiation (UV-B) is one of the most serious environmental stressors in embryonic development. Here, we investigated susceptibility to UV-B (0.5 kJ m⁻²) in embryos of the two-spotted spider mite, Tetranychus urticae, to examine the potential use of UV-B in control of this important agricultural pest worldwide. Peak susceptibility to UV-B (0% hatchability) was found in T. urticae eggs 36–48 h after oviposition at 25 °C, which coincides with the stages of morphogenesis forming the germ band and initial limb primordia. However, hatchability recovered to ~ 80% when eggs irradiated with UV-B were subsequently exposed to visible radiation (VIS) at 10.2 kJ m⁻², driving photoreactivation (the photoenzymatic repair of DNA damage). The recovery effect decreased to 40–70% hatchability, depending on the embryonic developmental stage, when VIS irradiation was delayed for 4 h after the end of exposure to UV-B. Thus UV-B damage to T. urticae embryos is critical, particularly in the early stages of morphogenesis, and photoreactivation functions to mitigate UV-B damage, even in the susceptible stages, but immediate VIS irradiation is needed after exposure to UV-B. These findings suggest that nighttime irradiation with UV-B can effectively kill T. urticae eggs without subsequent photoreactivation and may be useful in the physical control of this species.
Agid:
5965301