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The GlaA signal peptide substantially increases the expression and secretion of α-galactosidase in Aspergillus niger

Author:
Xu, Yue, Wang, Yan-hui, Liu, Tian-qi, Zhang, Hui, Zhang, He, Li, Jie
Source:
Biotechnology letters 2018 v.40 no.6 pp. 949-955
ISSN:
0141-5492
Subject:
Aspergillus niger, alpha-galactosidase, enzyme activity, genes, glucan 1,4-alpha-glucosidase, industrial applications, pH, secretion, signal peptide
Abstract:
OBJECTIVE: α-Galactosidases are widely used in many fields. It is necessary to improve the production of enzymes through microbiological processes. The aim of this study was to construct recombinant Aspergillus niger strains with high α-galactosidase production. RESULTS: Two recombinant A. niger strains were constructed: AB and AGB. The recombinant AB strain contained the α-galactosidase aglB gene from A. niger with its native AglB signal peptide regulated by the glucoamylase promoter. In the AGB recombinant strain, the AglB signal peptide was replaced with the glucoamylase (GlaA) signal peptide. The extracellular maximum α-galactosidase activity of the AGB strain was 215.7 U/ml and that of the AB strain was 9.8 U/mL. The optimal conditions for α-galactosidase were pH 3.5 and 35 °C. CONCLUSIONS: The GlaA signal peptide substantially increased the yield of secreted α-galactosidase in A. niger. This recombinant strain holds great potential for industrial applications.
Agid:
5965341