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Isolation of human mesenchymal stem cells from amnion, chorion, placental decidua and umbilical cord: comparison of four enzymatic protocols

Author:
Araújo, A.B., Furlan, J.M., Salton, G.D., Schmalfuss, T., Röhsig, L.M., Silla, L.M. R., Passos, E.P., Paz, A.H.
Source:
Biotechnology letters 2018 v.40 no.6 pp. 989-998
ISSN:
0141-5492
Subject:
amnion, chorion, collagenase, humans, protocols, stem cells, trypsin, umbilical cord
Abstract:
OBJECTIVE: To compare four enzymatic protocols for mesenchymal stem cells (MSCs) isolation from amniotic (A-MSC) and chorionic (C-MSC) membranes, umbilical cord (UC-MSC) and placental decidua (D-MSC) in order to define a robust, practical and low-cost protocol for each tissue. RESULTS: A-MSCs and UC-MSCs could be isolated from all samples using trypsin/collagenase-based protocols; C-MSCs could be isolated from all samples with collagenase- and trypsin/collagenase-based protocols; D-MSCs were isolated from all samples exclusively with a collagenase-based protocol. CONCLUSIONS: The trypsin-only protocol was least efficient; the collagenase-only protocol was best for C-MSCs and D-MSCs; the combination of trypsin and collagenase was best for UC-MSCs and none of tested protocols was adequate for A-MSCs isolation.
Agid:
5965345