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An efficient biocatalytic synthesis of imidazole-4-acetic acid

Author:
Zhao, Weirui, Ding, Huanru, Hu, Sheng, Huang, Jun, Lv, Changjiang, Mei, Jiaqi, Jin, Zhihua, Yao, Shanjing, Mei, Lehe
Source:
Biotechnology letters 2018 v.40 no.7 pp. 1049-1055
ISSN:
0141-5492
Subject:
Escherichia coli, Proteus vulgaris, biocatalysis, biocatalysts, cost effectiveness, histidine, hydrogen peroxide, synthesis
Abstract:
OBJECTIVE: To develop a new and efficient biocatalytic synthesis method of imidazole-4-acetic acid (IAA) from L-histidine (L-His). RESULTS: L-His was converted to imidazole-4-pyruvic acid (IPA) by an Escherichia coli whole-cell biocatalyst expressing membrane-bound L-amino acid deaminase (mL-AAD) from Proteus vulgaris firstly. The obtained IPA was subsequently decarboxylated to IAA under the action of H₂O₂. Under optimum conditions, 34.97 mM IAA can be produced from 50 mM L-His, with a yield of 69.9%. CONCLUSIONS: Compared to the traditional chemical synthesis, this biocatalytic method for IAA production is not only environmentally friendly, but also more cost effective, thus being promising for industrial IAA production.
Agid:
5965354