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Micropropagation of the Mongolian medicinal plant Zygophyllum potaninii via somatic embryogenesis

Bayarmaa, Gun-Aajav, Lee, NaNyum, Kang, HoDuck, Oyuntsetseg, Batlai, Moon, HeungKyu
Plant biotechnology reports 2018 v.12 no.3 pp. 187-194
2,4-D, Zygophyllum, abscisic acid, callus, cotyledons, culture media, endangered species, greenhouses, hypocotyls, medicinal plants, micropropagation, perlite, plantlets, sand, seed germination, somatic embryogenesis, somatic embryos, xerophytes
The Mongolian medicinal plant Zygophyllum potaninii has been assessed as an endangered species with regional status. We applied the somatic embryogenesis technique using aseptic in vitro germinants of the plant as an effective propagation technology. The seed germination rate in vitro was 16.5% after 2 weeks of culture. Embryonic calli (EC) and somatic embryos (SEs) were induced using the cotyledon or hypocotyl segments of the germinants. Calli were effectively induced on MS medium supplemented with 0.1 mg/L 2, 4-dichlorophenoxy acetic acid (2, 4-D) and 0.5 mg/L 6-benzylamino purine (BA). The callus was composed of pale yellow or pale green friable cells. SE formed from EC only on Murashige and Skoog medium (MS) with 0.5 mg/L abscisic acid (ABA). Other concentrations of ABA failed to induce SE formation. All SEs germinated in MS medium with different salt levels. However, normal plant conversion was achieved only on half-strength MS medium. The converted plantlets were effectively acclimatized in vitro in sand and transferred to a mixture of sand and perlite (1:1 v/v) in the greenhouse. After 8 weeks of culture, 55.4% of the plants survived. This is a first report of propagating the medicinal desert plant Z. potaninii via somatic embryogenesis and plant regeneration.