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Enhanced Production of β-D-glycosidase and α-L-arabinofuranosidase in Recombinant Escherichia coli in Fed-batch Culture for the Biotransformation of Ginseng Leaf Extract to Ginsenoside Compound K

Kim, Tae-Hun, Yang, Eun-Joo, Shin, Kyung-Chul, Hwang, Kyeong-Hwan, Park, JunSeong, Oh, Deok-Kun
Biotechnology and bioprocess engineering 2018 v.23 no.2 pp. 183-193
Escherichia coli, Panax, Sulfolobus solfataricus, Thermotoga, alpha-N-arabinofuranosidase, biological production, bioprocessing, biotransformation, cosmetics, dietary supplements, enzyme activity, fermenters, ginsenosides, ingredients, leaf extracts, leaves, traditional medicine
Ginsenoside compound K is an essential ingredient in nutritional supplements, cosmetics, and traditional medicines. However, cultivation for the production of enzymes involved in ginsenoside biotransformation has not been attempted in a fermenter. The host strain Escherichia coli ER2566 and the constitutive pHCE vector were selected for the efficient production of β-D-glycosidase, and expression medium composition to produce Sulfolobus solfataricus β-glycosidase expressed in E. coli was optimized in flask and batch cultures. The total activity of β-Dglycosidase in fed-batch culture using a fermenter increased 14-fold before optimization. S. solfataricus β-D-glycosidase and Thermotoga petrophila α-L-arabinofuranosidase were produced in a fed-batch culture. These two enzymes completely converted protopanaxadiol-type ginsenosides in ginseng leaf extract obtained from discarded ginseng leaves as a renewable substrate to compound K. The effective bioprocess for compound K production developed here will contribute to the industrial biological production of compound K.