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SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology

Author:
Abdelmoez, Mahmoud N., Iida, Kei, Oguchi, Yusuke, Nishikii, Hidekazu, Yokokawa, Ryuji, Kotera, Hidetoshi, Uemura, Sotaro, Santiago, Juan G., Shintaku, Hirofumi
Source:
Genome biology 2018 v.19 no.1 pp. 66
ISSN:
1474-760X
Subject:
cytoplasm, gene expression, messenger RNA, physiological state, sequence analysis
Abstract:
We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, nucRNA and cytRNA, per cell, quantifies gene expression in the subcellular compartments, and combines them to create novel single-cell RNA-seq data. Leveraging SINC-seq, we discover distinct natures of correlation among cytRNA and nucRNA that reflect the transient physiological state of single cells. These data provide unique insights into the regulatory network of messenger RNA from the nucleus toward the cytoplasm at the single-cell level.
Agid:
5967447