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Alginate-encapsulation of nodal segments of guava (Psidium guajava L.) for germplasm exchange and distribution
- Rai, Manoj K., Jaiswal, Vijai S., Jaiswal, Uma
- Journal of horticultural science & biotechnology 2008 v.83 no.5 pp. 569-573
- Psidium guajava, benzyladenine, biotechnology, calcium, calcium alginate, culture media, encapsulation, gels, genetically modified plants, genotype, germplasm exchange, guavas, horticulture, liquids, plantlets, sodium alginate
- Nodal segments obtained from in vitro grown plantlets of guava (Psidium guajava L.) were encapsulated in calcium alginate beads for germplasm exchange and distribution. The best gel complex for encapsulation of nodal segments was achieved using 3% (w/v) sodium alginate and 100 mM calcium chloride.The maximum conversion of encapsulated nodal segments into plantlets was obtained on growth regulator-free, full-strength, liquid Murashige and Skoog medium after a pulse treatment with 4.4 µM BAP (6-benzylaminopurine) for 1 week prior to encapsulation. Plants regenerated from encapsulated nodal segments were acclimatised successfully. The present encapsulation approach may also be useful in large-scale propagation of desirable elite genotypes and genetically modified plants.