U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Comparison of anti-Campylobacter activity of free thymol and thymol-β-d-glucopyranoside in absence or presence of β-glycoside-hydrolysing gut bacteria

Author:
Sharon V.R. Epps, Branko T. Petrujkić, Ivana Sedej, Nathan A. Krueger, Roger B. Harvey, Ross C. Beier, Thad B. Stanton, Timothy D. Phillips, Robin C. Anderson, David J. Nisbet
Source:
Food chemistry 2015 v.173 pp. 92-98
ISSN:
0308-8146
Subject:
Campylobacter coli, Campylobacter jejuni, antibacterial properties, cattle, cecum, coculture, enzyme activity, feces, fermentation, glucosides, glycosidases, intestinal absorption, intestinal microorganisms, swine, thymol, volatile fatty acids
Abstract:
Thymol is a natural product that exhibits antimicrobial activity in vitro but in vivo results indicate that absorption within the proximal alimentary tract precludes its delivery to the distal gut. Presently, the anti-Campylobacter activity of thymol was compared against that of thymol-β-d-glucopyranoside, the latter being resistant to absorption. When treated with 1mM thymol, Campylobacter coli and jejuni were reduced during pure or co-culture with a β-glycoside-hydrolysing Parabacteroides distasonis. Thymol-β-d-glucopyranoside treatment (1mM) did not reduce C. coli and jejuni during pure culture but did during co-culture with P. distasonis or during mixed culture with porcine or bovine faecal microbes possessing β-glycoside-hydrolysing activity. Fermentation acid production was reduced by thymol-β-d-glucopyranoside treatment, indicating that fermentation was inhibited, which may limit its application to just before harvest. Results suggest that thymol-β-d-glucopyranoside or similar β-glycosides may be able to escape absorption within the proximal gut and become activated by bacterial β-glycosidases in the distal gut.
Agid:
59711
Handle:
10113/59711