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Quickly verifying the antioxidant contribution of the individual composition in natural antioxidants by HPLC-free radical scavenging detection

Wang, Lu, Luo, You, Wu, Yanan, Xia, Fenggeng, Wu, Zhenqiang
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.96 pp. 461-468
2,2-diphenyl-1-picrylhydrazyl, antioxidant activity, antioxidants, cations, gallic acid, guavas, kaempferol, leaves, procyanidins, quercetin, tea
An HPLC-free radical-scavenging activity detection (HPLC-FRSAD) method was established to evaluate the antioxidant activity of each component in a mixed standard phenolics solution (MSPs). This method quickly displayed the differences in each composition in the MSPs with regard to their scavenging abilities by DPPH radical and ABTS radical cation. The relationship between the structures and the antioxidant activities of the compounds was discussed. Gallic acid, quercetin, and kaempferol in the MSPs’ compositions represented stronger scavenging activities of DPPH radical and ABTS radical cation. Furthermore, the developed method was successfully applied to screen major antioxidants from guava leaf tea extract (GLTE). The results revealed that the gallic acid, procyanidin B3, quercetin, and kaempferol in the extract had higher DPPH radical and ABTS radical cation scavenging activities than other components, which would be responsible for the antioxidant activities of GLTE. The present method provides a useful strategy for rapidly recognizing the key antioxidant components in complex natural products.