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Identification of novel QTL for leaf traits in soybean

Tae-Hwan JUN, Keith FREEWALT, Andy P. MICHEL, Rouf MIAN
Plant breeding 2014 v.133 no.1 pp. 61-66
Glycine max, crop yield, inbred lines, leaf area, leaves, quantitative trait loci, single nucleotide polymorphism, soybeans
Several leaf traits of soybean (Glycine max L. Merr.), including leaf area (LA), leaf shape (LS) and specific leaf weight (SLW) may be related to soybean yield. The objective of this study was to identify novel quantitative trait loci (QTL) for LA, LS and SLW in a recombinant inbred line (RIL) population developed from a cross of soybean cultivar Wyandot and PI 567301B. The phenotype data were collected in 2011 and 2012 for 93 F7-derived RILs and their parents using a randomized complete block design with 2-replicates each year. Five hundred sixteen single nucleotide polymorphism (SNP) markers generated earlier from this population and the phenotype data were used to detect QTL for LA, LS, and SLW using single marker analysis and interval mapping. Single markers analysis identified 26 QTL for the three traits of which 17 were novel and the rests were previously reported QTL. Most of these QTL were also identified by interval mapping, though the logarithm of odds (LOD) values for few QTL were below the significance threshold at P<0.05. As we have used a dense genetic map constructed with publicly available SNP markers, most QTL reported here were located in close proximity (<1 cM) of one or more SNP markers. These publicly available SNP markers with close linkage with LA, LS, and SLW are highly valuable for marker assisted breeding for these traits.