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Effect of Marek’s disease vaccines on interferon and toll like receptors when administered in ovo

Author:
Gimeno, Isabel M., Glaize, Ayanna, Cortes, Aneg L.
Source:
Veterinary immunology and immunopathology 2018 v.201 pp. 62-66
ISSN:
0165-2427
Subject:
Marek disease, chickens, embryo (animal), gene expression regulation, genes, immune response, interferon-alpha, interferon-beta, interferon-gamma, lungs, messenger RNA, receptors, spleen, thymus gland, transcription (genetics), vaccination, vaccines
Abstract:
The effect of two Marek’s disease (MD) vaccines on the chicken embryo immune responses were evaluated. Transcription of interferon (IFN-α, IFN-β, IFN-λ, and IFN-γ) and interferon-I receptors (IFN-AR1 and IFN-AR2), as well as transcription of toll like receptors (TLR-3, TLR-7, and TLR-21) were evaluated in the bursa, thymus, spleen and lung of 1-day-old chickens that had been vaccinated with HVT, CVI988, or sham inoculated at embryonic day 18 (ED18). Each vaccine had a unique effect on the transcription of the evaluated genes and it differs among tissues. HVT increased IFN-γ and TLR-3 transcripts in the spleen and lung and IFN-β in the bursa. The immune responses elicited by CVI988 differed from that observed in the HVT inoculated group. CVI988 downregulated several of the studied genes and only upregulated IFN-β and TLR-21 in spleen. Differences in vaccine replication (53% of spleens and lungs of HVT-vaccinated embryos but only 22% of spleens of CVI988-vaccinated embryos had detectable viral gB transcripts) were detected. Previously, we have shown that intra-amniotic vaccination at ED18 with HVT but not with CVI988 rendered chickens more immunocompetent at hatch. The role of increased transcription of TLR-3 and IFN-γ in such positive effect warrant further investigations.
Agid:
5972116