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Genetic analysis of resistance to six virus diseases in a multiple virus-resistant maize inbred line

Jose Luis Zambrano, Mark W. Jones, Eric Brenner, David M. Francis, Adriana Tomas, Margaret G. Redinbaugh
Theoretical and applied genetics 2014 v.12 no.4 pp. 867-880
Maize chlorotic dwarf virus, Maize dwarf mosaic virus, Maize fine streak virus, Maize mosaic virus, Sugarcane mosaic virus, Wheat streak mosaic virus, additive gene effects, corn, genes, genetic resistance, genetic techniques and protocols, hybrids, inbred lines, inheritance (genetics), loci, progeny, quantitative trait loci, viruses
Virus diseases in maize can cause severe yield reductions that threaten crop production and food supplies in some regions of the world. Genetic resistance to different viruses has been characterized in maize populations under diverse environments and screening techniques, and resistance loci have been mapped to all maize chromosomes. The maize inbred line, Oh1VI, is resistant to at least 10 viruses, including viruses in five different families. To determine the genes and inheritance mechanisms responsible for the multiple virus resistance in this line, F1 hybrids, F2 progeny and a recombinant inbred line (RIL) population derived from a cross of Oh1VI and the virus-susceptible inbred line Oh28 were evaluated. Progeny were screened for their responses to Maize dwarf mosaic virus, Sugarcane mosaic virus, Wheat streak mosaic virus, Maize chlorotic dwarf virus, Maize fine streak virus, and Maize mosaic virus. Depending on the virus, dominant, recessive, or additive gene effects were responsible for the resistance observed in the F1. For all viruses, one, two or three gene models explained the observed segregation of resistance in the F2 generation. Composite interval mapping in the RIL population identified 17 resistance QTLs associated with the six viruses. Of these, 15 were clustered in specific regions of chromosomes 2, 3, 6, and 10. It is unknown whether these regions of clustered QTLs contain single or multiple virus resistance genes, but the coupling phase linkage of genes conferring resistance to multiple virus diseases in this population could facilitate breeding efforts to develop multi-virus resistant crops.