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Influence of metal ions on biofilm formation by Arthrobacter sp. SUK 1205 and evaluation of their Cr(VI) removal efficacy

Dey, Satarupa, Paul, A.K.
International biodeterioration & biodegradation 2018 v.132 pp. 122-131
Arthrobacter, biofilm, biomass, chromium, confocal laser scanning microscopy, glass, magnesium, metal ions, mining, nickel, pH, salts, scanning electron microscopy, temperature, wastewater, India
This study explores biofilm formation by the chromate resistant and reducing Arthrobacter sp. SUK 1205 isolated from Sukinda chromite mining regions of Odisha, India. Formation of biofilm by the isolate was pronounced in complex peptone-yeast extract-glucose medium and was positively influenced by Cr(VI), Mg(II) and Ni(II). The optimum pH and temperature for biofilm formation was 7.0 and 37 °C, respectively. Architectural analysis following Scanning Electron Microscopy and Confocal Laser Scanning Microscopy revealed increase in total biomass, thickness and production of extracellular polymeric substances in the biofilm. Biofilms developed on glass beads under the influence of metal ions were used for Cr(VI) removal in synthetic Minimal salts (MS) medium and complete removal of 0.5 mM Cr(VI) was achieved within 4 days with chromium induced biofilms. Experiments were also conducted to reuse and recycle the biofilms to assess their Cr(VI) removal efficacy and also to evaluate their utility in removal of Cr(VI) from metal contaminated waste water.