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Effects of Eicosanoid Biosynthesis Inhibitors on the Prophenoloxidase Transcript Level in BombyxmoriL

Author:
Zhang, Congfen, Zhu, Baojian, Dai, Li shang, Liu, Chaoliang, Luo, Xuegang
Source:
Journal of the Kansas Entomological Society 2017 v.90 no.3 pp. 194-203
ISSN:
0022-8567
Subject:
biosynthesis, defense mechanisms, eicosanoids, environmental factors, gene expression, hemocytes, innate immunity, lipopolysaccharides, prophenoloxidase, signal transduction, silkworms
Abstract:
The silkworm, Bombyxmori L., has developed various defense mechanisms that reducethe negative effects of adverse environmental conditions. However, the mechanisms by which many physiological and ecological aspects work, including the functioning of the prophenoloxidase (pro-PO) system in Bombyxmori (BmPPO), are not completely understood. In our previous study, we found that eicosanoids mediated the innate immunity of the Chinese oak silkworm, Antheraeapernyi. To investigate the role of the pro-PO system in the silk-producing insect, Bombyxmori, the transcripts and activity of BmPPO were studied. We found that lipopolysaccharides (LPS) could induce the transcripts of BmPPO1, while eicosanoids could mediatethe induction of BmPPO1 gene expression in hemocytes. Most interestingly, the expression of BmPPO2 had no response to LPS challenge and treatment with eicosanoids. These results support the hypothesis that the B.mori pro-POsystem is associated with the eicosanoids signaling pathway.
Agid:
5975967