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Biodegradation of Pendimethalin by Paracoccus sp. P13

Ni, Haiyan, Li, Na, Qiu, Jiguo, Chen, Qing, He, Jian
Current microbiology 2018 v.75 no.8 pp. 1077-1083
alkanes, biodegradation, carbon, carbon dioxide, enzymatic reactions, nucleotide sequences, oxidation, pH, pendimethalin, phylogeny, physiology, ribosomal DNA, ribosomal RNA, soil, tandem mass spectrometry, temperature, ultra-performance liquid chromatography
In this study, a bacterial strain P13 capable of degrading pendimethalin was isolated from the soil of a fruit garden. Based on observed cellular morphology and physiology characteristics and a phylogenetic analysis of 16S rRNA gene sequences, strain P13 was identified as a member of the genus Paracoccus. Strain P13 grew on pendimethalin as the sole carbon source, and could degrade 100 mg/L pendimethalin within 2 days and 200 mg/L pendimethalin within 5 days. Pendimethalin degradation was proposed to be initiated by oxidation ring cleavage to yield 1,3-dinitro-2-(pentan-3-ylamino)butane-1,4-diol, an alkane organic compound that was identified by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS), which then underwent a series of enzymatic reactions to produce CO₂ and H₂O. The optimal pH and temperature for pendimethalin degradation by strain P13 were 7.0 and 30 °C, respectively. This study identified the bacterial strain Paracoccus sp. P13, which degraded pendimethalin with a relatively high efficiency, and presents a previously unreported microbial pendimethalin degradation pathway.