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Introgression of Gossypium barbadense L. into Upland cotton germplasm RMBUP-C4S1

Jenkins, Johnie N., McCarty, Jack C., Jr, Deng, Dewayne, Geng, Lige, Hayes, R. W., Jones, D. C., Mammadova, Ruhangiz
Euphytica 2018 v.214 no.7 pp. 118
Gossypium barbadense, Gossypium hirsutum, alleles, cotton, cultivars, fiber quality, germplasm, heat, highlands, introgression, loci, microsatellite repeats, plant breeding, population structure, random mating, self-pollination, substitution lines
Gossypium barbadense L. cotton has significantly better fiber quality than Upland cotton (G. hirsutum L.); however, yield and environmental adaptation of G. barbadense is not as wide as Upland. Most cotton in the world is planted to Upland cultivars. Many attempts have been made, over a considerable number of years, to introgress fiber quality alleles from G. barbadense into Upland. However, introgression barriers, primarily in the form of interspecific incompatibility, have limited these traditional approaches. The use of chromosome substitution lines (CSL) as a bridge should provide a more efficient way to introgress alleles from G. barbadense into Upland. We crossed 18 G. barbadense CSL to three cultivars and developed a random mated population. After five cycles of random mating followed by one generation of self-pollination to increase the seed supply, we grew the random mated population and used 139 G. barbadense chromosome specific SSR markers to assess a random sample of 96 plants for introgression. We recovered 121 of 139 marker loci among the 96 plants. The distribution of the G. barbadense alleles ranged from 10 to 28 alleles in each plant. Among the 96 plants we found individual plants with marker loci from 6 to 14 chromosomes or chromosome arms. Identity by descent showed little relatedness among plants and no population structure was indicated by a heat map. Using CSL we were able to develop a mostly Upland random mated population with considerable introgression of G. barbadense alleles which should be useful for breeding.