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Carbon-nanotube-caged microbial electrodes for bioelectrocatalysis

Author:
Xia, Hong-qi, Sakai, Kento, Kitazumi, Yuki, Shirai, Osamu, Takagi, Kazuyoshi, Kano, Kenji
Source:
Enzyme and microbial technology 2018 v.117 pp. 41-44
ISSN:
0141-0229
Subject:
Methylobacterium extorquens, atmospheric pressure, carbon dioxide, carbon nanotubes, catalytic activity, electrodes, formate dehydrogenase, formates, microorganisms, oxidation, pH, paraquat
Abstract:
A method to stably immobilize microbes on electrodes was developed. Resting cells of Methylobacterium extorquens AM1(MeAM1) were caged within multiwalled carbon nanotubes (MWNTs)by adding the cells to a water dispersion of MWNTs then allowing the resulting mixture to dry on electrodes. The MeAM1-MWCNTs electrode thus obtained displayed excellent activities in the bidirectional bioelectrocatalysis due to formate dehydrogenase(s) in the resting cells; formate oxidation and carbon dioxide reduction proceeded at steady-state catalytic current densities of 0.6 ± 0.1 and −0.8 ± 0.1 mA cm−2, respectively, using methyl viologen as mediator under very mild conditions (pH 7.0, atmospheric pressure, and 37 °C). In addition, the catalytic signal was stable for more than one week under continuous operation.
Agid:
5980324