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Characterization and hypoglycemic effect of a neutral polysaccharide extracted from the residue of Codonopsis Pilosula

Liu, Wei, Lv, Xing, Huang, Wenhua, Yao, Wenbing, Gao, Xiangdong
Carbohydrate polymers 2018 v.197 pp. 215-226
Codonopsis pilosula, Fourier transform infrared spectroscopy, arabinose, dispersibility, enzyme activity, ethanol, galactose, gas chromatography-mass spectrometry, glucose, glycemic effect, glycolysis, high performance liquid chromatography, insulin secretion, lipid metabolism, liver, methylation, mice, molecular weight, nuclear magnetic resonance spectroscopy, oxidative stress, particle size, polysaccharides, sodium sulfate, transmission electron microscopy
A neutral polysaccharide coded as CERP1 was extracted and purified from the residue of Codonopsis pilosula by ethanol precipitation and column chromatography. The structure of CERP1 was determined by HPAEC-PAD, HPLC, HPSEC-MALLS, FT-IR, NMR, and TEM. The results showed that CERP1 was a heteropolysaccharide composed of Arabinose, Glucose, and Galactose in the ratio of 1.00:19.83:6.94. HPSEC-MALLS showed that CERP1 was homogeneous with an absolute molecular weight of 4.840 × 103 Da. The NMR results and the GC–MS result of methylation indicated that 1-linked β-d-glucose, 1,3-linked β-d-glucose, 1,6-linked β-d-glucose and 1,3,6-linked β-d-galactose were the main linkages in CERP1. TEM results indicated that CERP1 had a homogeneous particle size and good dispersibility both in purified water and 0.05 M sodium sulfate. Pre-treatment with CERP1 could result in a significant improvement of the insulin secretion of the INS-1 cells. While in vivo assays, CERP1 exhibited a potent hypoglycemic effect on T2DM mice for its ability to relieve oxidative stress, ameliorate lipid metabolism, increase glycolytic enzyme activity as well as decrease liver transaminase activity. This study gives a chance for exploring the residue of Codonopsis pilosula.