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Characterisation of pectin-xylan complexes in tomato primary plant cell walls
- Broxterman, Suzanne E., Schols, Henk A.
- Carbohydrate polymers 2018 v.197 pp. 269-276
- EDTA (chelating agent), anion exchange chromatography, carrots, cell walls, cellulose, chelating agents, chemical bonding, coprecipitation, milling, pectins, solubilization, strawberries, tomatoes, uronic acids, xylan, xylose
- The primary plant cell wall is composed of a complex network of pectin, hemicellulose and cellulose. Potential interactions between these polysaccharides were studied for carrot, tomato and strawberry, with a focus on the role of pectin. The Chelating agent Unextractable Solids (ChUS), the residue after water- and EDTA extraction, was ball milled and subsequently water extracted. For tomato and strawberry, pectin and substantial amounts of hemicellulose were solubilised. Anion exchange chromatography (AEC) showed co-elution of pectin and acetylated glucuronoxylan in tomato, representing 18% of solubilised uronic acid and 48% of solubilised xylose by ball milling from ChUS. The existence of a covalently linked pectin-xylan complex was proposed since xylan co-precipitated with pectin under mild alkali conditions. It was proposed that pectin links with xylan through the RG-I region since degradation of HG did not alter AEC elution patterns for RG-I and xylan, suggesting RG-I – xylan interactions.