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Characterisation of pectin-xylan complexes in tomato primary plant cell walls

Broxterman, Suzanne E., Schols, Henk A.
Carbohydrate polymers 2018 v.197 pp. 269-276
EDTA (chelating agent), anion exchange chromatography, carrots, cell walls, cellulose, chelating agents, chemical bonding, coprecipitation, milling, pectins, solubilization, strawberries, tomatoes, uronic acids, xylan, xylose
The primary plant cell wall is composed of a complex network of pectin, hemicellulose and cellulose. Potential interactions between these polysaccharides were studied for carrot, tomato and strawberry, with a focus on the role of pectin. The Chelating agent Unextractable Solids (ChUS), the residue after water- and EDTA extraction, was ball milled and subsequently water extracted. For tomato and strawberry, pectin and substantial amounts of hemicellulose were solubilised. Anion exchange chromatography (AEC) showed co-elution of pectin and acetylated glucuronoxylan in tomato, representing 18% of solubilised uronic acid and 48% of solubilised xylose by ball milling from ChUS. The existence of a covalently linked pectin-xylan complex was proposed since xylan co-precipitated with pectin under mild alkali conditions. It was proposed that pectin links with xylan through the RG-I region since degradation of HG did not alter AEC elution patterns for RG-I and xylan, suggesting RG-I – xylan interactions.