Effects of the HN gene C-terminal extensions on the Newcastle disease virus virulence
- Source:
- Virus genes 2013 v.47 no.3 pp. 498-504
- ISSN:
- 0920-8569
- Subject:
- chickens, sequence analysis, open reading frames, chicken eggs, Avian orthoavulavirus 1, amino acids, genes, viruses, sialidase, death, eggs, virulence
- Abstract:
- The hemagglutinin-neuraminidase (HN) of Newcastle disease virus (NDV) is a multifunctional protein that has receptor recognition, neuraminidase, and fusion promotion activities. Sequence analysis revealed that the HN gene of many extremely low virulence NDV strains encodes a larger open-reading frame (616 amino acids, aa) with additional 45 aa at its C-terminus when compared with that (571 aa) of virulent NDV strains. Therefore, it has been suspected that the 45 aa extension at the C-terminus of the HN may affect the NDV virulence. In this study, we generated an NDV mesogenic strain Anhinga-based recombinant virus with an HN C-terminal extension of 45 aa (rAnh- HN-ex virus) using reverse genetics technology. The biological characterization of the recombinant virus showed that the rAnh-HN-ex virus had similar growth ability to its parental virus rAnh-wt both in embryonating chicken eggs and DF-1 cells. However, the pathogenicity of this recombinant virus in embryonating chicken eggs and day-old chickens decreased, as evidenced by a longer mean death time and lower intracerebral pathogenicity index when compared with the parental virus. This is consistent with our previous finding that the recombinant LaSota virus with a 45-aa extension at its HN C-terminal was attenuated in chickens and embryonating eggs. These results suggest that
- Agid:
- 59811
- Handle:
- 10113/59811
- https://doi.org/10.1007/s11262-013-0973-4