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Gas chromatography-mass spectrometry based plasma metabolomics of H1N1-induced inflammation in mice and intervention with Flos Lonicerae Japonica-Fructus Forsythiae herb pair

Qian, Wenjuan, Kang, An, Peng, Linxiu, Xie, Tong, Ji, Jianjian, Zhou, Wei, Shan, Jinjun, Di, Liuqing
Journal of chromatography 2018 v.1092 pp. 122-130
biochemical pathways, body weight, gas chromatography-mass spectrometry, histology, inflammation, interleukin-6, lungs, metabolites, metabolomics, mice, models, multivariate analysis, oral administration, tumor necrosis factor-alpha, viral pneumonia
Flos Lonicerae Japonica-Fructus Forsythiae herb pair (Yin-Qiao in Chinese, YQ), is used clinically for the treatment of viral pneumonia due to its heat-clearing and detoxifying functions. In the present work, the effect of YQ in H1N1-induced inflammation in mice was investigated by metabolomics based on GC–MS. Body weight and histological results were used to assess the lung injury, while the levels of IL-6 and TNF-α in plasma were used to evaluate the extent of inflammation. The acquired GC–MS data were further subjected to multivariate data analysis, and the significantly altered metabolites identified. After statistical and pathway analysis, 17 significantly altered metabolites and 3 possible metabolic pathways were found in plasma between normal and H1N1-induced pneumonia mice, while 17 significant differential metabolites were identified when YQ treatment group was compared with model group. This work indicates that oral administration of YQ could protect mice from H1N1-induced inflammation partially by ameliorating the associated metabolic disturbances.