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Isolation and structure determination of new saponins from Pulsatilla cernua based on an NMR-guided method and their anti-proliferative activities

Wang, An-dong, Bao, Ying, Liu, Dan, Wang, Xia, Li, Mei-chen, Liu, Jian-yu, Xu, Yong-nan
Phytochemistry letters 2018 v.27 pp. 9-14
Anemone cernua, Pulsatilla, cytotoxicity, human cell lines, humans, inhibitory concentration 50, isolation techniques, neoplasm cells, nuclear magnetic resonance spectroscopy, roots, saponins, spectral analysis
Based on an NMR-guided isolation method, three saponins with kalopanax-genin L1 as aglycone were isolated from the roots of Pulsatilla cernua. Among them, pulsatillacernuoside Ka (1) and pulsatillacernuoside Kb (2) are new compounds and their aglycone, kalopanax-genin L1, has never been reported from the genus Pulsatilla. Their structures were confirmed on the basis of spectroscopic data, including 2D NMR spectra and chemical evidence. In this study, all thirty-six compounds were investigated for the anti-proliferative activities against five human cancer cell lines (A549, MDA-MB-231, SK-Hep-1, SNU638, and HCT116). Among them, compound 2 exhibited moderate cytotoxicity to the MDA-MB-231 and SK-Hep-1, and the IC50 values are from 61.9 μM to 66.3 μM. By contrast, compound 25 exhibited significant cytotoxic activity against all cell lines A549 (IC50 = 3.8 μM), MDA-MB-231 (IC50 = 5.1 μM), SK-Hep-1 (IC50 = 1.8 μM), SNU638 (IC50 = 4.6 μM) and HCT116 (IC50 = 0.8 μM).