U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Main content area

Enterobacteriaceae andSalmonella recovered from nonsanitized and sanitized broiler hatching eggs

M.T. Musgrove, C.B. Stephens, D.V. Bourassa, N.A. Cox, J.M. Mauldin, M.E. Berrang, R.J. Buhr
Journal of applied poultry research 2014 v.23 no.3 pp. 516-522
Escherichia coli, Salmonella, bacteria, bacterial contamination, decontamination, eggs, flocks, foams, guanidines, hatching, pathogen survival, sanitizers, sanitizing, spraying
Sanitizing hatching eggs may reduce the chances that a broiler flock will become colonized withSalmonella and reduce the numbers of other microorganisms, such asEnterobacteriaceae, that can depress hatchability. An experiment was conducted to determine if a quaternary-biguanide sanitizer applied as foam or spray would reduceEnterobacteriaceae orSalmonella naturally occurring on broiler hatching eggs. The sanitizer was applied to buggies of 5,040 eggs the day before set (one buggy/treatment at each of 2 settings). Treated eggs were compared with untreated controls. Foam application loweredEnterobacteriaceae prevalence at set (0 vs. 18%) and transfer (5 vs. 28%); spraying was effective only when eggs were set (2.5 vs. 11%). At transfer spray, treated and control eggs were 19%Enterobacteriaceae-positive. FiveSalmonella-positives were recorded during the study. No indication that the sanitizer was effective in reducingSalmonella prevalence when applied as foam was observed (3/120 vs. 1/120). NoSalmonella were recovered from spray-treated eggs. No statistically significant difference forSalmonella prevalence was noted, but with such a low rate of recovery it is difficult to draw a firm conclusion. However, the sanitizer applied as foam was effective at decreasing the prevalence ofEnterobacteriaceae (a family of bacteria that includesSalmonella andEscherichia coli), and is present more often and in higher numbers thanSalmonella.