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Uninduced high-yield bacterial expression of fluorescent proteins

Author:
Sarabipour, Sarvenaz, King, Christopher, Hristova, Kalina
Source:
Analytical biochemistry 2014 v.449 pp. 155-157
ISSN:
0003-2697
Subject:
Escherichia coli, bacteria, biomedical research, cost effectiveness, fluorescent proteins
Abstract:
Here we introduce a fast, cost-effective, and highly efficient method for production of soluble fluorescent proteins from bacteria. The method does not require optimization and does not use isopropyl β-d-1-thiogalactopyranoside (IPTG) induction. The method relies on uninduced expression in the BL21–Gold (DE3) strain of Escherichia coli and yields large amounts (up to 0.4μmol) of fluorescent protein from a 250-ml culture. This method is much simpler than published methods and can be used to produce any fluorescent protein that is needed in biomedical research.
Agid:
5983890