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Sensitive Detection of Single-Cell Secreted H2O2 by Integrating a Microfluidic Droplet Sensor and Au Nanoclusters
- Shen, Rui, Liu, Peipei, Zhang, Yiqiu, Yu, Zhao, Chen, Xuyue, Zhou, Lu, Nie, Baoqing, Żaczek, Anna, Chen, Jian, Liu, Jian
- Analytical chemistry 2018 v.90 no.7 pp. 4478-4484
- cell movement, droplets, fluorescence, gold, horseradish, hydrogen peroxide, immunity, nanogold, nanoparticles, secretion
- As an important signaling molecule, hydrogen peroxide (H₂O₂) secreted externally by the cells influences cell migration, immunity generation, and cellular communications. Herein, we have developed a microfluidic approach with droplets in combination with Au nanoclusters for the sensitive detection of H₂O₂ secreted by a single cell. Isolated in the ultrasmall volume (4.2 nL) of a microdroplet, single-cell secreted H₂O₂ can initiate dramatic fluorescence changes of horseradish peroxidase–Au nanoclusters. We have demonstrated an ultrahigh sensitivity (200–400 attomole H₂O₂ directly measured from a single cell) with good specificity. It offers a useful research tool to study the cell-to-cell differences of H₂O₂ secretion at the single-cell level.