Main content area

Noninvasive Multimodal Imaging of Osteosarcoma and Lymph Nodes Using a 99mTc-Labeled Biomineralization Nanoprobe

Xu, Zhiming, Wang, Yangyun, Han, Jianshan, Xu, Qingqing, Ren, Jiawei, Xu, Jiaying, Wang, Yong, Chai, Zhifang
Analytical chemistry 2018 v.90 no.7 pp. 4529-4534
biocompatibility, biomineralization, fluorescence, image analysis, lymph nodes, magnetic resonance imaging, mice, models, osteosarcoma, planning, resection
The accurate imaging of the lymph nodes represents a critical indicator for tumor staging and surgical planning (e.g., osteosarcoma). Clinically, nodal tracing using a radio-nanocolloid is often limited by the inaccessibility of real-time images and inadequate anatomical information. Herein, we present a ⁹⁹ᵐTc-labeled biomineralization nanoprobe for the advanced detection of osteosarcoma and lymph nodes with multimodal imaging. Through the exploitation of the complementary strengths of MRI/SPECT/NIR fluorescence, the fabricated nanoprobe exhibited suitable stability and biocompatibility characteristics and was shown to be able to be located in osteosarcoma. The lymphatic drainage and network in healthy mice were imaged in real-time using NIR fluorescence and SPECT/CT. Furthermore, we demonstrated that our ⁹⁹ᵐTc-biomineralization nanoprobe could be used for the high-resolution and high-sensitivity imaging analysis of lymphatic drainage in an orthotopic osteosarcoma model. Overall, the ⁹⁹ᵐTc-labeled biomineralization nanoprobe features promising characteristics to be used as an intraoperative visualization tool to aid in precise tumor imaging and nodal resection.