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In Vivo Real-Time Monitoring System Using Probe Electrospray Ionization/Tandem Mass Spectrometry for Metabolites in Mouse Brain
- Zaitsu, Kei, Hayashi, Yumi, Murata, Tasuku, Yokota, Kazumi, Ohara, Tomomi, Kusano, Maiko, Tsuchihashi, Hitoshi, Ishikawa, Tetsuya, Ishii, Akira, Ogata, Koretsugu, Tanihata, Hiroshi
- Analytical chemistry 2018 v.90 no.7 pp. 4695-4701
- agonists, cannabinoids, cerebrum, energy metabolism, ionization, metabolites, mice, monitoring, spectrometers, tandem mass spectrometry
- Recent improvements in ambient ionization techniques combined with mass spectrometry has enabled to achieve real-time monitoring of analytes of interest, even for biogenic molecules in living animals. Here, we demonstrate a newly developed system for in vivo real-time monitoring of metabolites in a living mouse brain. It consists of a semiautomated manipulation system and a unique probe electrospray ionization unit, which uses an extremely thin solid needle (tip dia.: 700 nm) for direct sampling and ionization, coupled to a conventional tandem mass spectrometer. The system successfully monitored 8 cerebrum metabolites related to central energy metabolism in an isoflurane-anesthetized mouse in real time with a 20 s interval. Moreover, our system succeeded in capturing dynamics of energy metabolism in a mouse administered with cannabinoid type-1 receptor agonist, which is known to disrupt cerebrum energy metabolism. The present system now opens the door to the next stage of cutting-edge technique in achieving in vivo real-time monitoring.