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Rapid Quantitative Profiling of Lipid Oxidation Products in a Food Emulsion by 1H NMR

Author:
Merkx, Donny W. H., Hong, G. T. Sophie, Ermacora, Alessia, van Duynhoven, John P. M.
Source:
Analytical chemistry 2018 v.90 no.7 pp. 4863-4870
ISSN:
1520-6882
Subject:
aldehydes, emulsions, fatty acids, hydroperoxides, isomers, lipid peroxidation, mayonnaise, models, nuclear magnetic resonance spectroscopy, oxidation, shelf life, temperature
Abstract:
Lipid oxidation is one of the most important reasons for the compromised shelf life of food emulsions. A major bottleneck in unravelling the underlying mechanisms is the lack of methods that provide a rapid, quantitative, and comprehensive molecular view on lipid oxidation in these heterogeneous systems. In this study, the unbiased and quantitative nature of ¹H NMR was exploited to assess lipid oxidation products in mayonnaise, a particularly oxidation-prone food emulsion. An efficient and robust procedure was implemented to produce samples where the ¹H NMR signals of oxidation products could be observed in a well resolved and reproducible manner. ¹H NMR signals of hydroperoxides were assigned in a fatty acid and isomer specific way. Band-selective ¹H NMR pulse excitation allowed immediate and precise (RSDR = 5.9%) quantification of both hydroperoxides and aldehydes with high throughput and large dynamic range at levels of 0.03 mmol/kg. Explorative multivariate data modeling of the quantitative ¹H NMR profiles revealed that shelf life temperature has a significant impact on lipid oxidation mechanisms.
Agid:
5986436