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Improving the efficiency of plasmid transformation in Shewanella oneidensis MR-1 by removing ClaI restriction site

Author:
Rachkevych, Nazarii, Sybirna, Kateryna, Boyko, Solomiya, Boretsky, Yuriy, Sibirny, Andriy
Source:
Journal of microbiological methods 2014 v.99 pp. 35-37
ISSN:
0167-7012
Subject:
Shewanella oneidensis, genetic transformation, plasmids
Abstract:
Here we demonstrate that elimination of ClaI restriction site from the sequence of a plasmid DNA increases the efficiency of transformation of Shewanella oneidensis MR-1 significantly. To achieve reliable transformation of S. oneidensis MR-1 plasmids either lacking ClaI site or isolated from primary transformants of S. oneidensis should be used.
Agid:
5998695