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Genome-wide association and validation of key loci for yield-related traits in wheat founder parent Xiaoyan 6

Author:
Ma, Feifei, Xu, Yunfeng, Ma, Zhengqiang, Li, Lihui, An, Diaoguo
Source:
Molecular breeding 2018 v.38 no.7 pp. 91
ISSN:
1380-3743
Subject:
Triticum aestivum, agronomic traits, alleles, breeding programs, genetic markers, genome-wide association study, genotyping, grain yield, growing season, inbred lines, loci, marker-assisted selection, microsatellite repeats, parents, phenotypic variation, plant height, polymerase chain reaction, quantitative trait loci, seeds, spikelets, wheat, China
Abstract:
Xiaoyan 6, one of the most important founder parents in wheat, possesses many superior agronomic traits and has played a crucial role in Chinese wheat breeding programs. In this study, a panel of 66 elite wheat accessions derived from Xiaoyan 6 was planted in four growing seasons; genome-wide association study (GWAS) was performed for six yield-related traits using the wheat 90K genotyping assay. A total of 803 significant marker-trait associations (MTAs) that explained up to 35.0% of the phenotypic variation were detected. Of these, the locus QTkw-5B which contains 19 MTAs for thousand kernel weight (TKW) was consistently detected in three growing seasons and confirmed in a recombinant inbred line (RIL) population by developing simple sequence repeats (SSR) and kompetitive allele-specific PCR (KASP) markers. The locus QPh-3A containing eight repetitive MTAs for plant height (PH) was consistently identified in all the four growing seasons and validated in a RIL population by developing SSR markers. The transmission of Xiaoyan 6 allele indicated that the favorite allele of QPh-3A was strongly selected in breeding programs. Comparing with previous studies, QTkw-5B and QPh-3A should be novel QTL. The locus QFss-2D for fertile spikelet number per spike (FSS) was identified and then validated in three bi-parental populations. This locus controlled various spike-related traits and may be a key spike polymorphic locus. This study could provide insight into dissecting yield-related traits in the breeding population and reliable molecular markers that might be valuable for marker-assisted selection in wheat high-yield breeding programs.
Agid:
6000798