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A novel QTL qSPP2.2 controlling spikelet per panicle identified from Oryza longistaminata (A. Chev. et Roehr.), mapped and transferred to Oryza sativa (L.)

Kaur, Amanpreet, Sidana, Kannu, Bhatia, Dharminder, Neelam, Kumari, Singh, Gurpreet, Sahi, Gurpreet K., Gill, Baljeet K., Sharma, Priti, Yadav, Inderjit S, Singh, Kuldeep
Molecular breeding 2018 v.38 no.7 pp. 92
5' untranslated regions, Oryza longistaminata, Oryza sativa, alleles, chromosome mapping, chromosomes, grain yield, microsatellite repeats, panicles, phenotypic variation, quantitative trait loci, sequence analysis, spikelets, tillers, wild relatives, wild rice, yield components
Increasing the rice productivity from the current 10 to 12 tons/ha to meet the demand of estimated 8.8 billion people in 2035 is posing a major challenge. Wild relatives of rice contain some novel genes which can help in improving rice yield. Spikelet per panicle (SPP) is a valuable trait for determining yield potential in rice. In this study, a major QTL for increasing SPP has been identified, mapped, and transferred from African wild rice O. longistaminata to O. sativa (L.). The QTL was mapped on the long arm of chromosome 2 in a 167.1 kb region flanked by SSR markers RM13743 and RM13750, which are 1.0 cM apart, and is designated as qSPP2.2. The QTL explained up to 30% of phenotypic variance in different generations/seasons and showed positive additive effect of allele contributed by O. longistaminata. In addition, O. longistaminata allele in qSPP2.2 contributed to increase in grains per panicle, but decrease in the tillers per plant. The 167.1 kb region contains 23 predicted genes. Based on the functional annotation, three genes, LOC_Os02g44860, LOC_Os02g44990, and LOC_Os02g45010, were selected as putative candidates for characterization. Sequence analysis of the three genes revealed functional variations between the parental lines for LOC_Os02g44990 and a variation in 5′UTR for LOC_Os02g45010 which will help further to identify putative candidate gene(s). This is the first yield component QTL to be identified, mapped, and transferred from O. longistaminata.