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Determination and identification of hydrophilic and hydrophobic arsenic species in methanol extract of fresh cod liver by RP-HPLC with simultaneous ICP-MS and ESI-Q-TOF-MS detection

Author:
Arroyo-Abad, Uriel, Lischka, Susanne, Piechotta, Christian, Mattusch, Jürgen, Reemtsma, Thorsten
Source:
Food chemistry 2013 v.141 no.3 pp. 3093-3102
ISSN:
0308-8146
Subject:
arsenic, arsenicals, chemical structure, cod (fish), fatty acids, hydrocarbons, liver, mass spectrometry, methanol, molecular weight, reversed-phase high performance liquid chromatography, taxonomy
Abstract:
The present study was focused on the determination and identification of arsenic species in methanolic extracts of cod liver. Arsenic species were fractionated and the fractions analysed by RP-HPLC-ICP-MS coupled with ESI-Q-TOF-MS. The total concentration of arsenic in the fresh cod liver was analysed by ICP-MS to be 1.53±0.02mgAskg−1w.w. and the extraction recovery was ca. 100% and the column recovery >93%. Besides polar inorganic and methylated arsenic species (>70%) more hydrophobic arsenic-containing fatty acids and hydrocarbons occurred. Based on the mass spectrometric data proposals for molecular structures were elaborated for 20 of the organic As species included 10 arsenic-containing fatty acids (AsFA) and an arsenic-containing hydrocarbon (AsHC) mentioned for the first time in fresh cod liver. Arsenobetaine was found as main water-soluble arsenic compound in cod liver followed by higher molecular mass arsenic-containing fatty acids and hydrocarbons.
Agid:
600948