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The somatic proteins of Toxocara canis larvae and excretory-secretory products revealed by proteomics

da Silva, Márcia B., Urrego A., Juan R., Oviedo, Yisela, Cooper, Philip J., Pacheco, Luis G.C., Pinheiro, Carina S., Ferreira, Fátima, Briza, Peter, Alcantara-Neves, Neuza M.
Veterinary parasitology 2018 v.259 pp. 25-34
Toxocara canis, Toxocara cati, cats, diagnostic techniques, dogs, excretory-secretory products, host-parasite relationships, immune response, larvae, mass spectrometry, metabolism, parasites, polyacrylamide gel electrophoresis, proteomics, tissues, toxocariasis, vaccines, zoonoses
Toxocariasis is a widespread helminth infection of dogs and cats, caused by Toxocara canis and Toxocara cati larvae, respectively. Toxocara spp. can cause zoonotic infections in humans by invading tissues and organs causing pathology. Toxocara spp. larvae release excretory-secretory molecules (TES) into the body of their host that are fundamental to the host-parasite interaction and could be used as targets for novel diagnostics and vaccines. In the present study, we identified 646 T. canis proteins from TES and larval extract using 1D-SDS PAGE followed by mass spectrometry. A wide range of proteins was identified that may play a role both in the induction of the host immune response and host pathology, and in parasite metabolism and survival. Among these proteins there are potential candidates for novel diagnostics and vaccines for dogs and cats toxocariases.