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Interaction dynamics between endophytic biocontrol agents and pathogen in the host plant studied via quantitative real-time polymerase chain reaction (qPCR) approach

Author:
Chow, Yiing Yng, Rahman, Sadequr, Ting, Adeline Su Yien
Source:
Biological control 2018 v.125 pp. 44-49
ISSN:
1049-9644
Subject:
DNA, Diaporthe phaseolorum, Elaeis guineensis, Ganoderma boninense, Penicillium citrinum, Trichoderma asperellum, biological control, biological control agents, clones, endophytes, host plants, leaves, models, monitoring, pathogens, quantitative polymerase chain reaction, tissues
Abstract:
The interaction dynamics between endophytic biocontrol agents and pathogens were investigated to determine their co-existence in the host plant. The endophytes (Diaporthe phaseolorum WAA02, Trichoderma asperellum T2, Penicillium citrinum BTF08) and pathogen (Ganoderma boninense Gb) were inoculated into oil palm ramets and their relative abundance in root, stem and leaf tissues was assessed via quantitative real-time PCR. This was done using single inoculations (for every endophyte and pathogen) and co-inoculations (combined inoculation of every endophyte with Gb). In single inoculations, BTF08 (P. citrinum) was the most abundant in the host tissues (3.93 × 1018 ng/mg DNA concentration), followed by WAA02 (D. phaseolorum) and T2 (T. asperellum) (1.30 × 103 and 6.48 × 101 ng/mg, respectively). Pathogenic Gb was the least abundant (1.58 × 101 ng/mg). When co-inoculated with Gb, all endophytic isolates outcompete Gb suggesting superiority of endophytic isolates when in co-existence with Gb. All isolates favoured colonization in stem tissues (0.024–4.84 × 1017 ng/mg), followed by root (0.045–7.30 × 1015 ng/mg) and leaf tissues (0.072–1.16 × 104 ng/mg). This study represents the first report on the simultaneous monitoring of pathogen and endophytic BCA in a host plant model via real-time PCR.
Agid:
6015814