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Circadian gene period1b regulates proinflammatory cytokine expression through NF–κB signalling in zebrafish

Ren, Da-long, Wang, Xiao-bo, Hu, Bing
Fish & shellfish immunology 2018 v.80 pp. 528-533
Danio rerio, circadian clocks, fish, gene expression, gene expression regulation, genes, immune response, immune system, interleukin-6, larvae, mitogen-activated protein kinase, models, mutants, mutation, phosphorylation, protein content, shellfish, tail, transcription factor NF-kappa B, tumor necrosis factor-alpha
The circadian clock plays a critical role in regulating the immune system. Our previous publication revealed that a mutation in the circadian gene period1b (per1b) in zebrafish significantly decreased proinflammatory gene expression, particularly under constant darkness (DD) conditions; however, the underlying mechanisms remain unclear. In this study, using per1b-null mutant zebrafish and a larval tail fin injury model, we observed that the loss of per1b resulted in the downregulation expression of proinflammatory cytokines, such as IL-6 and TNF-α, at protein level. Furthermore, the loss of per1b downregulated ERK phosphorylation and inhibited p65 phosphorylation, leading to reduced NF-κB activation, which could downregulate the expression of proinflammatory cytokines, such as IL-6 and TNF-α, in zebrafish. These results provided insight into the communication between the circadian clock and immune functions.