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Flavipin in Chaetomium globosum CDW7, an endophytic fungus from Ginkgo biloba, contributes to antioxidant activity

Ye, Yonghao, Xiao, Yu, Ma, Liang, Li, Hongxia, Xie, Zhenglu, Wang, Minghua, Ma, Haitian, Tang, Huaiwu, Liu, Junyan
Applied microbiology and biotechnology 2013 v.97 no.16 pp. 7131-7139
Chaetomium globosum, Ginkgo biloba, Nematoda, antioxidant activity, antioxidants, correlation, endophytes, fermentation, fungi, internal transcribed spacers, metabolites, ribosomal DNA, screening, therapeutics
1,2-Benzenedicarboxaldehyde-3,4,5-trihydroxy-6-methyl (flavipin) was found to be antagonistic against nematodes and fungi. Here we demonstrated that flavipin is a potent antioxidant in vitro and in vivo, which has great potential in the therapy for free radical-associated diseases. Therefore, flavipin-producing bio-source was screened from 80 endophytes in Ginkgo biloba. Seven endophytic fungi were able to synthesize antioxidant substances and identified by ITS rDNA sequences. Among them, Chaetomium globosum CDW7 was a remarkable producer of flavipin. The fermentation parameters of CDW7 were then optimized for high flavipin production. Cultured under the optimal condition (25 °C, 100/250 mL flask, 12 discs/flask, 150 rpm, pH 6.5) for 14 days, CDW7 was able to synthesize flavipin at a production of 315.5 mg/L. In addition, flavipin output was positively correlated to antioxidant activities of crude extracts with a correlation coefficient of 0.8235, indicating that flavipin was the major antioxidant component of CDW7's metabolites. These data demonstrated that CDW7 was a highly yielded bio-source of antioxidant flavipin.