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Purification, characterization and antioxidant activity of dextran produced by Leuconostoc pseudomesenteroides from homemade wine

Author:
Du, Renpeng, Qiao, Xiaoxiao, Zhao, Fangkun, Song, Qiaozhi, Zhou, Qingqing, Wang, Yu, Pan, Lei, Han, Ye, Zhou, Zhijiang
Source:
Carbohydrate polymers 2018 v.198 pp. 529-536
ISSN:
0144-8617
Subject:
2,2-diphenyl-1-picrylhydrazyl, Fourier transform infrared spectroscopy, Leuconostoc pseudomesenteroides, antioxidant activity, dextran, emulsifying properties, ethanol, exopolysaccharides, food industry, gas chromatography, gel chromatography, glucose, hydroxyl radicals, iron, molecular weight, nuclear magnetic resonance spectroscopy, temperature, thermal stability, water holding capacity, water solubility, wines
Abstract:
An exopolysaccharide (EPS) was produced by Leuconostoc pseudomesenteroides DRP-5 isolated from homemade wine. The EPS was obtained with ethanol extraction, which was further purified by chromatography of Sephadex G-100 to get a purified fraction. The monosaccharide composition of the EPS was glucose, and its molecular weight (Mw) was 6.23 × 106 Da, as determined by gas chromatography (GC) and high-performance size-exclusion chromatography (HPSEC). Fourier transform infrared spectra (FT-IR) and nuclear magnetic resonance spectra (NMR) showed that the EPS was a linear glucan with α-(1→6)-linked glucosidic bonds. The water holding capacity (WHC), water solubility index (WSI) and emulsifying activity (EA) of DRP-5 EPS were 296.76 ± 18.93%, 98.62 ± 3.57% and 87.22 ± 2.18%, respectively. DRP-5 EPS have a higher degradation temperature of 278.36 °C, suggesting high thermal stability of the EPS. Also, DRP-5 EPS was found to have moderate 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, superoxide anion radical, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical, Fe2+ scavenging activities and reducing power. All these characteristics suggest that DRP-5 EPS might have potential applications in the pharmaceutical, cosmetic and food industries.
Agid:
6018460