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Enhanced cytotoxic and apoptotic potential in hepatic carcinoma cells of chitosan nanoparticles loaded with ginsenoside compound K

Author:
Zhang, Jianmei, Wang, Yijun, Jiang, Yunyao, Liu, Tingwu, Luo, Yanyan, Diao, Enjie, Cao, Yufeng, Chen, Liang, Zhang, Liang, Gu, Qian, Zhou, Jinyi, Sun, Fengting, Zheng, Wancai, Liu, Jianxun, Li, Xueqin, Hu, Weicheng
Source:
Carbohydrate polymers 2018 v.198 pp. 537-545
ISSN:
0144-8617
Subject:
antineoplastic activity, apoptosis, chitosan, cytotoxicity, dose response, drug carriers, drug therapy, drugs, fluorescence, human cell lines, humans, image analysis, inhibitory concentration 50, liver neoplasms, nanoparticles, neoplasm cells, surfactants, water solubility
Abstract:
Ginsenoside compound K (CK) has been shown to exhibit anticancer properties. In this study, chitosan nanoparticles loaded with ginsenoside compound K (CK-NPs) were prepared as a delivery system using a self-assembly technique with amphipathic deoxycholic acid-O carboxymethyl chitosan as the carrier, which improved the water solubility of CK. By evaluating drug loading, entrapment efficiency, and in vitro release behavior, the feasibility of CK-NPs as a drug carrier nanoparticle for the treatment of human hepatic carcinoma cells (HepG2) was investigated. Result revealed that CK and CK-NPs showed a dose-dependent inhibitory effect on HepG2 cells with IC50 values of 23.33 and 16.58 μg/mL, respectively. Furthermore, fluorescence imaging demonstrated that CK-NPs promoted cellular uptake in vitro. Therefore, all results indicated that CK-NPs might be a novel drug delivery system to improve the solubility and enhance the cytotoxic and apoptotic potentials of CK for effective liver cancer chemotherapy.
Agid:
6018464