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Effect of yeast extract addition to a mineral salts medium containing hydrolyzed plant xylan on fungal pullulan production

Kennedy II, Daniel E., West, Thomas P.
Zeitschrift für Naturforschung 2018 v.73 no.7-8 pp. 319-323
Aureobasidium pullulans, Spartina pectinata, ammonium sulfate, biomass production, grasses, hydrolysates, pullulan, sodium chloride, xylan, yeast extract, yeasts
The ability of the fungus Aureobasidium pullulans ATCC 42023 to produce pullulan from yeast extract-supplemented xylan hydrolysates of the prairie grass prairie cordgrass was examined relative to polysaccharide and cell biomass production, yield, and pullulan content of the polysaccharide. A pullulan concentration of 11.2 g L⁻¹ and yield of 0.79 g g⁻¹ was produced by ATCC 42023 when grown for 168 h at 30°C on the phosphate-buffered hydrolysate supplemented with yeast extract. The highest biomass level being 8.8 g L⁻¹ was produced by ATCC 42023 after 168 h on a yeast extract-supplemented, hydrolysate-containing complete medium lacking sodium chloride. The highest pullulan content of the polysaccharide produced by ATCC 42023 after 168 h on the hydrolysate medium supplemented with yeast extract and ammonium sulfate was 70%. The findings indicate that a polysaccharide with a high pullulan content can be produced at a relatively high yield by the fungus grown on a yeast extract-supplemented xylan hydrolysate, suggesting that pullulan could be produced using a biomass-based process.