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Effect of yeast extract addition to a mineral salts medium containing hydrolyzed plant xylan on fungal pullulan production
- Kennedy II, Daniel E., West, Thomas P.
- Zeitschrift für Naturforschung 2018 v.73 no.7-8 pp. 319-323
- Aureobasidium pullulans, Spartina pectinata, ammonium sulfate, biomass production, grasses, hydrolysates, pullulan, sodium chloride, xylan, yeast extract, yeasts
- The ability of the fungus Aureobasidium pullulans ATCC 42023 to produce pullulan from yeast extract-supplemented xylan hydrolysates of the prairie grass prairie cordgrass was examined relative to polysaccharide and cell biomass production, yield, and pullulan content of the polysaccharide. A pullulan concentration of 11.2 g L⁻¹ and yield of 0.79 g g⁻¹ was produced by ATCC 42023 when grown for 168 h at 30°C on the phosphate-buffered hydrolysate supplemented with yeast extract. The highest biomass level being 8.8 g L⁻¹ was produced by ATCC 42023 after 168 h on a yeast extract-supplemented, hydrolysate-containing complete medium lacking sodium chloride. The highest pullulan content of the polysaccharide produced by ATCC 42023 after 168 h on the hydrolysate medium supplemented with yeast extract and ammonium sulfate was 70%. The findings indicate that a polysaccharide with a high pullulan content can be produced at a relatively high yield by the fungus grown on a yeast extract-supplemented xylan hydrolysate, suggesting that pullulan could be produced using a biomass-based process.