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Efficiency of Lactic Acid Bacteria Strains for Decontamination of Aflatoxin M₁ in Phosphate Buffer Saline Solution and in Skimmed Milk

Bovo, Fernanda, Corassin, Carlos H., Rosim, Roice E., de Oliveira, Carlos A. F.
Food and bioprocess technology 2013 v.6 no.8 pp. 2230-2234
Bifidobacterium animalis, Lactobacillus delbrueckii, Lactobacillus rhamnosus, aflatoxins, buffers, decontamination, lactic acid bacteria, phosphates, skim milk, temperature, washing
The aim of this study was to evaluate the ability of seven lactic acid bacteria (LAB) strains to remove aflatoxin M1 (AFM1) in phosphate buffer saline (PBS) and in skimmed milk samples. The mean AFM1 removal by LAB in PBS ranged from 5.60 ± 0.45% to 45.67 ± 1.65% (n = 3). Heat-killed cells showed AFM1 removal percentages significantly higher than viable cells in contact times of 15 min or 24 h, although there were no significant differences between those times. AFM1/LAB complex resulted from tests with PBS was unstable, as 40.57 ± 4.66% to 87.37 ± 1.82% of AFM1 retained by bacteria were recovered in solution after washing with PBS. Heat-killed cells of Lactobacillus rhamnosus, Lactobacillus delbrueckii spp. bulgaricus, and Bifidobacterium lactis had the highest percentage (>33%) of AFM1 removal in PBS tests. In ultrahigh temperature (UHT) skimmed milk spiked with AFM1, the three selected LAB strains showed no significant differences in removing AFM1 at 37 °C, and only B. lactis had greater ability to remove AFM1 at 4 °C. Results demonstrated that AFM1 removal by LAB has a potential application to reduce toxin concentrations until safe levels in milk. © 2012 Springer Science+Business Media, LLC.