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Effect of Mixed Electron Donors on Autotrophic Denitrification by Pseudomonas sp. SZF15
- Su, Junfeng, Luo, Xianxin, Huang, Tinglin, Ma, Fang, Zheng, Shengchen, Shao, Sicheng
- Industrial & engineering chemistry process design and development 2017 v.56 no.7 pp. 1723-1730
- Pseudomonas, denitrification, gas chromatography, groundwater, iron, manganese, nitrates, nitrogen, pH, process design, temperature
- In this study, mixed electron donors of Mn(II) and Fe(II) were tested for their effects on autotrophic denitrification. The denitrification performance based on various Mn(II)/Fe(II) molar ratios was assessed via batch experiments conducted under different conditions of temperature, pH, and electron donor/electron acceptor molar ratios. Complete nitrate removal was found at high Mn(II)/Fe(II) molar ratios, 30 °C, pH 7.0, and an electron donor/electron acceptor molar ratio of 3.00. Furthermore, we assessed the expression of nirS through amplification approaches and determined N₂ levels by gas chromatography. Our results demonstrated that mixed electron donors are efficient for autotrophic denitrification, and strain SZF15 is a suitable candidate for the simultaneous removal of nitrogen, Fe(II), and Mn(II) in groundwater treatment. The present work will benefit the development of autotrophic denitrification technology.