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Altered gene regulation and potential association with metabolic resistance development to imidacloprid in the tarnished plant bug, Lygus lineolaris

Zhu, Yu Cheng, Luttrell, Randall
Pest management science 2015 v.71 no.1 pp. 40-57
Lygus lineolaris, cotton, complementary DNA, gene overexpression, drugs, microarray technology, monitoring, digestion, genes, imidacloprid, pests, phosphotransferases (kinases), insecticide resistance, gene expression regulation, esterases, adenosinetriphosphatase
BACKGROUND: Chemical spray on cotton is almost an exclusive method for controlling tarnished plant bug (TPB), Lygus lineolaris. Frequent use of imidacloprid is a concern for neonicotinoid resistance in this key pest. Information of how and why TPB becomes less susceptible to imidacloprid is essential for effective monitoring and managing resistance. RESULTS: Microarray analysis of 6688 genes in imidacloprid‐selected TPB (Im1500FF) revealed 955 upregulated and 1277 downregulated (≥twofold) genes in Im1500FF, with 369 and 485 of them annotated. Five P450 and nine esterase genes were significantly upregulated, and only one esterase gene and no P450 genes were downregulated. Other upregulated genes include helicases, phosphodiesterases, ATPases and kinases. Pathway analyses identified 65 upregulated cDNAs that encode 51 different enzymes involved in 62 different pathways, including P450 and esterase genes for drug and xenobiotic metabolisms. Sixty‐four downregulated cDNAs code only 17 enzymes that are associated with only 23 pathways mostly related to food digestion. CONCLUSIONS: This study demonstrated a significant change in gene expression related to metabolic processes in imidacloprid‐selected TPB, resulting in overexpression of P450 and esterase genes for potential excess detoxification and cross/multiple resistance development. The identification of these and other enzyme genes establishes a foundation to explore the complicity of potential imidacloprid resistance in TPB. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.