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Structural characterization and gastroprotective property of a novel glucofructan from Allium ampeloprasum var. porrum

Author:
Malafaia, Camila R.A., da Silva, Bernadete P., Tinoco, Luzineide W., Parente, José P.
Source:
Carbohydrate research 2015 v.402 pp. 44-49
ISSN:
0008-6215
Subject:
Allium porrum, chromatography, fructose, glucofructan, glucose, mass spectrometry, methylation, models, molecular weight, nuclear magnetic resonance spectroscopy
Abstract:
A new polysaccharide with an estimated weight-average molar mass of 2.6×10³ was isolated from Allium ampeloprasum var. porrum by hot water extraction, and purified by Sephacryl S-300 HR high-resolution chromatography. It was composed of d-fructose and d-glucose in 10:6 molar ratio, respectively. The structure of the glucofructan was investigated by chemical and spectroscopic methods, including methylation analysis, nuclear magnetic resonance, and electrospray mass spectrometry (ES-MS). The results permitted the structure of the glucofructan to be written as α-d-Glcp-(1→1)-β-d-Fruf-(2→1)-{[α-d-Glcp-(1→6)-β-d-Fruf-(2→6)]-β-d-Fruf-(2→1)}4-β-d-Fruf-(2↔1)-α-d-Glcp. Results of the present study indicated that this new glucofructan exhibited significant gastroprotective property, using in vivo experimental models.
Agid:
6021976