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Structure of the O-specific polysaccharide from the legume endosymbiotic bacterium Ochrobactrum cytisi strain ESC1T

Author:
Pac, Malgorzata, Komaniecka, Iwona, Zamlynska, Katarzyna, Turska-Szewczuk, Anna, Choma, Adam
Source:
Carbohydrate research 2015 v.413 pp. 37-40
ISSN:
0008-6215
Subject:
O-antigens, Ochrobactrum, bacteria, chemical analysis, legumes, moieties, nuclear magnetic resonance spectroscopy
Abstract:
The O-specific polysaccharide was obtained from the lipopolysaccharide of the legume-endosymbiotic bacterium Ochrobactrum cytisi strain ESC1ᵀ and studied by chemical analyses and 1D and 2D NMR spectroscopy. The polysaccharide was found to have a disaccharide repeating unit containing α-d-fucose and β-N-acetyl-d-galactosamine residues connected via (1→3)-glycosidic bonds, resulting in the following structure:→3)-α-d-Fucp-(1→3)-β-d-GalpNAc-(1→The d-GalpNAc residue was nonstoichiometrically substituted with a 4-O-methyl group (∼10%) or with a 4,6-O-(1-carboxy)-ethylidene residue (pyruvyl group) (∼10%).
Agid:
6022104