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Simultaneous analysis of coccidiostats and sulphonamides in non-target feed by HPLC-MS/MS and validation following the Commission Decision 2002/657/EC Part A Chemistry, analysis, control, exposure & risk assessment

Gavilán, Rosa Elvira, Nebot, Carolina, Patyra, Ewelina, Miranda, Jose Manuel, Franco, Carlos Manuel, Cepeda, Alberto
Food additives & contaminants 2018 v.35 no.6 pp. 1093-1106
analytical methods, coccidiostats, dairy farming, feed mills, feeds, sulfonamides
Taking into consideration the maximum level (ML) for coccidiostats included in the European Regulation 574/2011 and the fact that the presence of residues of sulphonamides in non-target feed is forbidden, the aim of this article is to present an analytical method based on HPLC-MS/MS for the identification and quantification of sulphonamides and coccidiostats in non-target feeds. The method was validated following Commission Decision 2002/657/EC, and recovery, repeatability and reproducibility were within the limits established in the Decision. For coccidiostats, the decision limit and detection capability were calculated for the different species taking into account the ML allowed in Regulation 574/2011. The applicability of the method was investigated in 50 feed samples collected from dairy farms, 50 obtained from feed mills and 10 interlaboratory feed samples.