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Studies on the bovine major histocompatibility class I and class II antigens using homozygous typing cells and antigen‐specific BoT4⁺ blast cells
- ROTHEL, J. S., DUFTY, J. H., WOOD, P. R.
- Animal genetics 1990 v.21 no.2 pp. 141-148
- antigen-presenting cells, antigens, cattle, genes, herds, loci, lymphocytes, sires
- Animals were identified from two sire lines as being homozygous for the class I bovine lymphocyte antigen (BoLA‐A) w23. These animals were also shown to be homozygous for class II antigens (BoLA‐D) which, however, differed between the two sire lines. Lymphocytes from these animals were then used either as stimulator cells in one‐way mixed lymphocyte reactions (MLR) with all animals in the herd carrying the w23 antigen or as antigen presenting cells to bovine T4⁺ cell blasts. It was shown that, within each sire Une, the genes encoding the MHC class I and class II antigens were closely linked. There were no detected recombinations between the MHC class I and class II regions nor within the BoLA‐D region responsible for mixed lymphocyte reactivity. MLR typing of MHC class II antigens correlated with the results from T‐lymphocyte proliferation studies. Cells from these cattle, which are homozygous at the class I and II MHC loci but differ in the class II antigen expressed, could be used to type the BoLA‐D of other cattle.