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Evaluation of Two Immunomagnetic Separation Techniques for the Detection and Recovery of Escherichia coli O157:H7 from Finished Composts

Author:
Reynnells, Russell, Callahan, Mary Theresa, Handy, Eric T., Roberts, Cheryl, Felton, Gary, Ingram, David T., Millner, Patricia D., Sharma, Manan
Source:
Food analytical methods 2014 pp. 3
ISSN:
1936-9751
Subject:
Escherichia coli O157, composts, immunomagnetic separation, microbial detection, polymerase chain reaction
Abstract:
Two rapid immunomagnetic separation (IMS) protocols were evaluated to recover inoculated E. coli O157:H7 (1-2 log CFU/g) from 30 different commercial, finished compost samples. Both protocols detected E. coli O157:H7 in compost samples; PCR techniques required the removal of inhibitors to reduce possibility of a false negative result.
Agid:
60251